Abstracto

Genotyping and phylogenic relationship of giardia isolates from Sudanese suspected patients using (TPI) triose phosphate isomerase gene.

Anas M Elnazeer, Samah Hassan Suliaman, Mubarak Mustafa, Ishraga Adam Elzain, Shafag Hassan Kirkusawi

Giardia lamblia is one of the most common intestinal pathogens in both humans and animals throughout the world, globally, there are greater than 700,000 deaths per year associated with diarrheal disease, and understanding the organism requires more in-depth knowledge of its biology. The current study amid to establish a research platform for the molecular characterization of Giardia isolates in Sudan, focusing on the biology of giardia, through, molecular characterization attempts to genotyping A laboratory-based experimental study was designated. A total of 30 fecal samples of giardia positive from suspected patients after informed consent were used in this study from three states. Molecular characterization of giardia isolates which included DNA extraction by Guanidine HCL after liquid nitrogen treatment. PCR detection and sequencing were obtained. PCR results showed that only 5 samples were positive out of 30 that represent 17% total of cyst samples when using tpi gene 605 bp, in the Sequencing results only two samples were successfully sequenced (one from Khartoum and one from Sinnar states) while bioinformatics results showed similarity between this isolates through alignment and multiple alignments to giardia in Africa. After sequencing of tpi gene, the Sudanese isolates from Khartoum state were found to be identical to genotype (A) with 80%, while samples from Sinnar State were identical to genotype (B) with 90%.