Abstracto

miR-499 rs3746444 polymorphism is associated with colorectal cancer susceptibility: A case-control study in a Chinese population

Jue Wang, Hong-Gang Yu

Background: Previous study suggested that miR-499 rs3746444 may contribute to the risk and prognosis of Colorectal Cancer (CRC). However, other studies did not support association between rs3746444 and CRC risk. Therefore, we did this study to assess whether miR-499 rs3746444 affect the risk of CRC.

Method: A total of 913 subjects were included in this case-control study, including 434 patients with CRC and 479 healthy controls. The miR-499 rs3746444 was genotyped using the TaqMan methodology in 96-well plates and read with the Sequence Detection Software (SDS, version 1.4) on an Applied Biosystems (ABI) 7500 Real-Time PCR System.

Results: The miR-499 rs3746444 polymorphism showed significant difference between CRC patients and healthy controls in genotype comparison (TC vs. TT: OR = 1.37, 95% CI 1.01-1.88, P = 0.04; CC vs. TT: OR = 3.18, 95% CI 1.83-5.55, P<0.01; CC+TC vs. TT: OR = 1.64, 95% CI 1.22-2.24, P<0.01). Additionally, the miR-499 rs3746444 T allele was significantly associated with CRC risk (OR=2.89, 95% CI 1.69-5.09, P<0.01).

Conclusion: The results of our study suggested that miR-499 rs3746444 was significantly associated with CRC risk.