Abstracto
Metabolic profiling of elite athletes with different cardiovascular demand
Mohamed A Elrayess
Background: Intensive exercise of elite athletes results in physiological changes in response to increased cardiovascular demand. This study aims to profile metabolic changes in elite athletes from different sport disciplines. Methods: Metabolic profiling of serum samples from 500 elite athletes from different sports disciplines who participated in national or international sports events and tested negative for doping abuse at anti-doping laboratories, was performed using non-targeted metabolomics-based spectroscopy combined with ultrahigh-performance liquid chromatography. Multivariate analysis was conducted using orthogonal partial method of least squares discriminant analysis. Differences in metabolic levels between athletes with varying cardiovascular demands were assessed by univariate linear models. Results: Out of 743 analyzed metabolites, 112 novel metabolites that changed significantly with increased cardiovascular demand were detected. These included markers of carboxylic acid beta oxidation, oxidative stress and energy-related metabolites. GGM sub-networks identified 6 subnetworks that captured the main metabolic pathways perturbed in reference to cardiovascular demand including fatty acids beta oxidation. Conclusion: Data provide evidence that athletes with high cardiovascular demand exhibit a definite metabolic profile which will reflect a singular life style characterized by a strict exercise and a special diet. Metabolic signatures related to elite athletes could potentially be used as biomarkers for his or her overall health and response to their strict environment.
Intensive exercise of elite athletes can cause physiological alterations within the circulatory system in response to increased stroke volume and vital sign , known collectively as cardiovascular demand (CD). This study aimed to match metabolic differences in elite athletes with high vs low/moderate CD and to reveal their underlying metabolic pathways as potential biomarker signatures for assessing health, performance, and recovery of elite athletes. Metabolic profiling of serum samples from 495 elite athletes from different sport disciplines (118 high CD and 377 low/moderate CD athletes) was conducted using non-targeted metabolomics-based spectroscopy combined with ultra-high-performance liquid chromatography. Results show that DAGs containing arachidonic were enriched in high CD along side branched-chain amino acids, plasminogens, phosphatidylcholines, and phosphatidylethanolamines, potentially indicating increased risk of disorder within the high CD group. Gamma-glutamyl amino acids and glutathione metabolism were increased in low/moderate CD group, suggesting more efficient oxidative stress scavenging mechanisms than the high CD group. This first most comprehensive metabolic profiling of elite athletes provides an evidence that athletes with different CD show a singular metabolic signature that reflects energy generation and oxidative stress and potentially places the high CD group at a better risk of disorder . Further studies are warranted for confirmation and validation of findings in other sport groups in light of potential confounders related to limited available information about participants.