Abstracto

Influence of anti-infection therapy on changes of dendritic cells in rabbits with buccal VX2 cancer accompanied by infectious inflammation

Nini Zhang, Zhihong Chen, Lin Zhang, Guilin Huang, Xiaohua Hu, Jie Yi, Li Yao, Ligang Zhang

Background: This study aimed to investigate the influence of anti-infection therapy on the HLA-DR and CD86 expression, maturation and function of the dendritic cells in rabbits with buccal VX2 cancer accompanied by infectious inflammation.

Methods: Inoculation of VX2 cancer cells, mechanical trauma, and intake of high glucose viscous milk were administered to induce buccal VX2 cancer and wound infection in rabbits. Animals were divided into 3 groups: Group A: buccal cancer rabbits with infectious inflammation received intramuscular injection of procaine and penicillin as well as intragastrical injection of tinidazole for consecutive 3 days; Group B: buccal cancer rabbits with infectious inflammation received intragastrical and intramuscular injection of normal saline for 3 days; Group C: buccal cancer rabbits without infection received intragastrical and intramuscular injection of normal saline for 3 days. The Peripheral Blood Mononuclear Cells (PBMCs) were incubated with the supernatant from cancers at different statuses to induce their differentiation into DCs.

Results: Flow cytometry showed the expression of CD86 and HLA-DR was the highest in Group C and the lowest in Group B in PBMCs after incubation with the supernatant from cancers, and the expression of CD86 and HLA-DR was the highest in Group A and the lowest in Group B in cancer tissues.

Conclusion: Anti-infection therapy can significantly increase the expression of CD86 and HLA-DR (markers of DCs) in the buccal cancer rabbits with infectious inflammation and is helpful for the control of infection in the cancer as well as for the maturation and function of DCs.