Abstracto

Design and Use of a Flexible RNAi Expression Vector in Mammalian Cells .

Fleur Delacour

We present pRIGHT11, a flexible mammalian expression vector for the generation of short interfering RNA (siRNA) in cells. To stimulate the expression of short siRNA, this vector uses opposing eukaryotic RNA polymerase III promoters U6 and H1. We've shown that pRIGHT11-derived siRNA can inhibit transfected reporter genes and endogenous genes in a sequencespecific manner. This retrovirus-based vector can also carry a random library of siRNA, allowing for quick identification of novel genes involved in specific cellular responses.