Abstracto

Components of electrophoresis and their principles.

Nahid Shimei*

The movement and separation of charged particles (ions) caused by an electric field is referred to as electrophoresis. Two electrodes (anode, cathode) with opposing charges are joined by a conducting liquid known as an electrolyte to form an electrophoretic system. Southern blotting is an electrophoretic separation method created by E.M. Southern. 77 A DNA sample is broken down into fragments using restriction enzymes, and the resulting material is then electrophoresed. After that, the DNA is moved to a stable support and hybridised there.