Abstracto

Chicken immunologically active proteins for the development of anti-idiotypic vaccines.

Ioana Manea

This study investigates the use of the chicken and egg system for the development of an immune response against antimicrobial resistance (AMR) bacteria. Methodology: Stage I: Brown leghorn chickens were immunized with I-spga immunogen which contained antigens from more than 20 inactivated AMR bacteria. Indirect ELISA was used to measure anti-bacterial antibody titers in the watery soluble fraction of eggs up to 14 weeks after the third immunization. Stage II: chicken groups have been formed that have individually consumed yolk or white egg from either hiperimune eggs or from eggs produced by unimmunized chicken. At the end of the experiment, presence of antibodies against original AMR bacteria was checked by ELISA in blood samples and eggs of birds used in the Stage II of the experiment.