Abstracto

Characterization of three-dimensional bone constructs derived from unloaded human fetal osteoblasts exposed to the random positioning machine

Alamelu Sundaresan

Human cells exposed to microgravity form large 3D tissue constructs mirroring the in vivo architecture (e.g. cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human fetal osteoblasts (hFOB 1.19) cells to the Random Positioning Machine (RPM) for 7 and 14 days with the purpose to engineer 3D bone constructs. RPM-exposure of hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, ECM and 3D multicellular spheroid (MCS) formation.