Abstracto

Association of Cathepsin G gene polymorphism in chronic periodontitis.

C Prathiba Reichal, R Sankari Malaiappan, A Paramasivam

Periodontitis is an inflammatory disease with the underlying etiology that is unknown. Cathepsin is well known genes that play important roles in periodontitis by interacting with autophagy-associated molecules in pathogenesis. Several studies have established that the role of Cathepsin G (CTSG) gene is a bacterial serine protease stored in the neutrophil azurophilic granules and the mutation of this gene established correlation between many other diseases like osteomyelitis and alzheimer’s diseases. Hence, the aim of the current study was to assess the association between Chronic Periodontitis (CP) and polymorphism of CTSG. Gingival tissue and blood samples are collected from both the control and case group and DNA isolation was done. CTSG N125S polymorphism was genotyped in 30 patients with CP and 30 healthy controls. DNA was obtained from patients with CP and controls. PCR amplification and Sequencing of Cathepsin G gene were completed and the data was analysed. The CTSG N125S genotype frequency was calculated. The percentage of occurrence of heterogeneous diseased mutation genes were found to be 1.67% and 5% respectively. The genotype frequency was found to be 0.77 in CP patients and 0.93 in controls. There was no statistically difference between CP patients and healthy controls. The study concludes that CTSG N125S polymorphism was not associated with CP. However, further studies have to be conducted with more samples to identify the correlation between chronic periodontitis and polymorphism of Cathepsin G gene.